diff --git a/src/flask/config.py b/src/flask/config.py
index 34ef1a57..9e37f2bd 100644
--- a/src/flask/config.py
+++ b/src/flask/config.py
@@ -293,7 +293,7 @@ class Config(dict):  # type: ignore[type-arg]
             with open(filename, "r" if text else "rb") as f:
                 obj = load(f)
         except OSError as e:
-            if silent and e.errno in (errno.ENOENT, errno.EISDIR):
+            if silent and e.errno in (errno.ENOENT, errno.EISDIR, errno.ENOTDIR):
                 return False
 
             e.strerror = f"Unable to load configuration file ({e.strerror})"
diff --git a/tests/test_config.py b/tests/test_config.py
index e5b1906e..2b5342b4 100644
--- a/tests/test_config.py
+++ b/tests/test_config.py
@@ -195,6 +195,19 @@ def test_config_missing_file():
     assert not app.config.from_file("missing.json", load=json.load, silent=True)
 
 
+def test_config_from_file_not_a_directory(tmp_path):
+    """from_file with silent=True returns False when a path component is not a
+    directory, matching the behavior of from_pyfile."""
+    file_in_tmp = tmp_path / "not_a_dir"
+    file_in_tmp.write_text("{}")
+    app = flask.Flask(__name__)
+    app.config.root_path = str(tmp_path)
+    # not_a_dir/config.json: not_a_dir is a file, not a directory
+    assert not app.config.from_file(
+        "not_a_dir/config.json", load=json.load, silent=True
+    )
+
+
 def test_custom_config_class():
     class Config(flask.Config):
         pass