diff --git a/src/flask/config.py b/src/flask/config.py
index 34ef1a57..9e37f2bd 100644
--- a/src/flask/config.py
+++ b/src/flask/config.py
@@ -293,7 +293,7 @@ class Config(dict):  # type: ignore[type-arg]
             with open(filename, "r" if text else "rb") as f:
                 obj = load(f)
         except OSError as e:
-            if silent and e.errno in (errno.ENOENT, errno.EISDIR):
+            if silent and e.errno in (errno.ENOENT, errno.EISDIR, errno.ENOTDIR):
                 return False
 
             e.strerror = f"Unable to load configuration file ({e.strerror})"
diff --git a/tests/test_config.py b/tests/test_config.py
index e5b1906e..cacefde1 100644
--- a/tests/test_config.py
+++ b/tests/test_config.py
@@ -195,6 +195,19 @@ def test_config_missing_file():
     assert not app.config.from_file("missing.json", load=json.load, silent=True)
 
 
+def test_config_enotdir_silent(tmp_path):
+    """from_file with silent=True should not raise when a path component
+    is a file instead of a directory (ENOTDIR)."""
+    # Create a regular file where a directory is expected in the path.
+    not_a_dir = tmp_path / "not_a_dir"
+    not_a_dir.write_text("")
+    app = flask.Flask(__name__)
+    app.config.root_path = str(tmp_path)
+    assert not app.config.from_file(
+        os.path.join("not_a_dir", "config.json"), load=json.load, silent=True
+    )
+
+
 def test_custom_config_class():
     class Config(flask.Config):
         pass